International Journal of Nano Dimension (Int. J. Nano Dimens.) Preparation and characterization of friendly colloidal Hydroxyapatite based on natural Milk’s casein Volume 9 (2018) Issue 3, August 2018 2024 02 28 Preparation and characterization of friendly colloidal Hydroxyapatite based on natural Milk’s casein EN Babak Sadeghi Department of Chemistry, Islamic Azad University, Tonekabon Branch, Tonekabon, IRAN. 0000-0003-0933-5782 Ali Akbar Sajjadi Institute of Water and Energy, Sharif University of Technology, Tehran, Iran Nazanin Farhadyar Department of Chemistry, Islamic Azad University, Varamin-Pishva, Iran. Babak Sadeghi Department of Chemistry, Islamic Azad University, Tonekabon Branch, Tonekabon, IRAN. 0000-0003-0933-5782 Journal Article 2024 02 28 Biocompatible hydroxyapatite nanocomposites are biocompatible, biodegradable and nontoxic have been paid many attentions as one of the most suitable vehicle for drug delivery use. Our objective in this work was to prepare and characterize caseins based HA nanocomposite in a colloidal form for drug delivery purposes. Casein biopolymer was firstly extracted from skimmed milk by adding acetic acid and was used then to prepare colloidal hydroxyapatite nanoparticles (nHA) through a modified microemulsion technique. Characterization of the as prepared samples was carried out by means of Fourier Transform Infrared (FT-IR) spectroscopy, X-ray powder diffraction patterns (XRD), Field Emission Scanning and Transmission Microscopy (FESEM & TEM).  Dynamic light scattering (DLS) technique was finally used to measure particle size and zeta potential distribution of the sample using electrophoresis mobility. The results revealed successful preparation of a colloidal HA /casein suspension with a hydrodynamic zeta average particle size distribution of 256.6 nm.