International Journal of Nano Dimension (Int. J. Nano Dimens.) Synthesis of MnFe2O4 nanoparticles for adsorption of digestive enzymes: Kinetics, isothermal and thermodynamics studies Volume 10 (2019) Issue 4, October 2019 2024 02 07 Synthesis of MnFe2O4 nanoparticles for adsorption of digestive enzymes: Kinetics, isothermal and thermodynamics studies EN Adeogun Abideen Idowu Chemistry Department, Federal University of Agriculture, Abeokuta, Nigeria. Kareem Sarafadeen Olateju Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria. Adebayo Oluwatobi Samson Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria. Journal Article 2024 02 07 The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of amylase and protease on to manganese ferrite (MnFe2O4). The metal ferrite was synthesized via a sol – gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnetic resonance (EPR) and Fourier transform infrared spectroscopy (FTIR). The adsorption was study in a batch process and the data were subjected to kinetics and isotherm models. The pseudo-first order best fitted the kinetic data with R2> 0.99. The data were fitted well by the entire isotherm models considered with the maximum adsorption isotherm of 1.602 and 7.330 mg/g.  The thermodynamic parameters give negative Gibb’s free energy, ΔG, showing a spontaneous adsorption; positive ΔH indicated an endothermic favoured process, while ΔS values showed that the process progress with lower entropy change.