International Journal of Nano Dimension (Int. J. Nano Dimens.) Electrospun biocompatible Gelatin-Chitosan/Polycaprolactone/Hydroxyapatite nanocomposite scaffold for bone tissue engineering Volume 10 (2019) Issue 2, April 2019 2024 02 07 Electrospun biocompatible Gelatin-Chitosan/Polycaprolactone/Hydroxyapatite nanocomposite scaffold for bone tissue engineering EN Samira Arabahmadi Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 0000-0002-4202-9931 Mohammad Pezeshki Modaress Burn Research Canter, Iran University of Medical Sciences, Tehran, Iran. Shiva Irani Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Mojgan Zandi Department of Biomaterial, Iran Polymer and Petrochemical Institute, Tehran, Iran. Journal Article 2024 02 07 In recent years, nanocomposite scaffolds made of bioactive polymers have found multiple applications in bone tissue engineering. In this study composite nanofibrous structure of gelatin (Gel)/chitosan (Cs)-polycaprolactone (PCL) containing hydroxyapatite (HA) were fabricated using co-electrospinning process. To assay the biocompatibility and bioactivity of electrospun nanocomposite scaffolds, the behavior of human osteosarcoma cells (MG63) on fabricated nanofibers was evaluated using scanning electron microscopy (SEM), fluorescence microscopy analysis, measuring calcium deposits and MTT assay. The SEM micrographs at days 3 and 7 showed high cell attachment and spreading on the nanofibrous scaffolds. The MTT results demonstrated the proliferation of MG-63 cells during 10 days and the positive effect of nanofibers in comparison of cell culture plate. Considering the proliferation rate and calcification extent, the Gel-Cs-HA nanofibers reveal highest biocompatibility for osteoblast cells which could be attributed to the smaller diameter fibers and more mechanical strength in the Gel-Cs-HA scaffold.