Anthropogenic Pollution Effects of Fe2o3 and Co2o3 nanoparticles on Organisms in Freshwater Volume 4 (2020) Issue 2, September 2020 2023 12 19 Effects of Fe2o3 and Co2o3 nanoparticles on Organisms in Freshwater 10.22034/AP.2020.1903672.1070 EN Leila Farsi Department of Environmental Science, Ahvaz Branch, Islamic Azad University, Ahvaz , Iran Mojgan Khodadadi Department of Aquaculture , Ahvaz Branch, Islamic Azad University, Ahwaz, Iran Sima Sabzalipour Department of Environmental Science, Ahvaz Branch, Islamic Azad University, Ahvaz , Iran Nemaat Jaafarzadeh Haghighi Fard Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Department of Environmental science Ahvaz Branch Islamic Azad university Ahvaz Iran Farid Jamali-Sheini Advanced Surface Engineering and Nano Materials Research Center, Department of Physics, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Journal Article 2023 12 19 Nanoparticles (NPs) are causing threats to the environment. In this review, we examined how hematite (Fe2O3) and cobalt oxide (Co2O3) nanoparticles impact the species of freshwater green algae Chlorella vulgaris (C. vulgaris). We exposed laboratory cultures to five initial concentrations of nanoparticles and measured impacts on species in 24, 48, 72, 96, 120, and 144 hours in Karun River water at 20-25°C. Our results indicated that Fe2O3 and Co2O3 NPs significantly (dependent on concentration) reduced the chlorophyll a, b, and carotenoid contents of algae C. vulgaris compared to the control group (P <0.05). Also, due to the combination of these two nanoparticles, Co2O3 (50 Fe2O3 +100 Co2O3) has a more negative effect on algae chlorophyll change. According to the data, the exposure concentration was also found to be a more effective factor in the Chlorophyll content in algae species as compared to the exposure time. Our study suggests this nanoparticle has potential to affect aquatic life and ecosystem properties of freshwater habitats.